Fusobacterium nucleatum의 테트라사이클린 내성여부를 검사하기 위한 세균배양법과 중합효소연쇄반응법의 비교
Comparison of cultural method and PCR method for the assay of tetracycline-resistant Fusobacterium cleatum isolated in Korean
최재흥, 장현규, 성진효, 이순례,
소속 상세정보
최재흥 ( ) - 조선대학교 치과대학 예방치학교실
장현규 ( ) - 조선대학교 치과대학 구강생화학교실
성진효 ( ) - 조선대학교 치과대학 예방치학교실
이순례 ( ) - 조선대학교 치과대학 예방치학교실
KMID : 0355420030270040581
Abstract
The purposes of this study were to identify the detection and incidence of tetracycline resistance determinant tet(M) by PCR method and to evaluate the availability of PCR method guided for clinical use of antibiotics in dentistry. Subgingival plaque samples were collected from 172 sites of 29 patients. Both original sample and subcultured sample, PCR amplification was used to detect tet(M) in Fusobacterium nucleatum. The percentage of tet(M)-positive subjects and sites´ in original samples were 100%, 95.9%, respectively. And the percentage of tet(M)-positive subjects and sites in subcultured samples were 17.2%, 3.5%, respectively. In subcultured samples, probing pocket depth and clinical attachment level in tet(M)positive sites were higher than in negative sites (p < 0.05). The sensitivity and specificity of PCR method compared with broth dilution method as a gold standard were 0.91 and 0.86, respectively. The results suggested that tet(M) assay using PCR method was available to detect the tetracycline-resistant F. nucleatum
키워드
Fusobacterium nucleatum;테트라사이클린내성;세균배양법;중합효소연쇄반응법;Cultural method;PCR method;tetracycline-resistant
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